Composite
Part:BBa_K3059234:Design
Designed by: Beteel Abu-Ageel Group: iGEM19_William_and_Mary (2019-10-21)
3G compatible LasI (LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 57
Design Notes
Used sticky ends C and D to ensure that this part would come together with an RBS that also had a C prefix sticky end, and a terminator that had a D suffix sticky end.
Source
Pseudomonas aeruginosa
Sources: 1. Karig, D., Martini, K. M., Lu, T., Delateur, N. A., Goldenfeld, N., & Weiss, R. (2018). Stochastic Turing patterns in a synthetic bacterial population. Proceedings of the National Academy of Sciences, 115(26), 6572-6577. doi:10.1073/pnas.1720770115
2. Karig, D. K. (2007). Engineering multi-signal synthetic biological systems. (Doctoral dissertation). Princeton University, USA.